nebuilder hifi dna assembly (New England Biolabs)
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Nebuilder Hifi Dna Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebuilder+hifi+dna+assembly+master+mix/NEBuilder+HiFi+DNA+Assembly+Master+Mix/bio_rxiv__64898__2026__10__15__759383-62-4-4
Average 99 stars, based on 9454 article reviews
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other:Article Title: Decoding how RNase E membrane targeting controls RNA degradosome activity in Pseudomonas aeruginosa Article Snippet: Amplification:Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by Construct:Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by Article Title: Myopathy-causing variants of PYROXD1 converge on impaired RNA ligation Article Snippet: .. Constructs were assembled from gel-purified PCR products or from synthesized DNA fragments using DNA Sequencing:Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by Synthesized:Article Title: Solute selectivity and functional divergence of aquaporins in reef-building corals Article Snippet: Full-length cDNAs encoding Acropora millepora Aqps (AmiAqps) were chemically synthesized ( Table S1 ; Eurofins Genomics, Tokyo, Japan) with 15 bp terminal sequences homologous to the ends of the linearized pGEMHE vector ( ). .. The synthesized cDNAs were subcloned into pGEMHE using the Article Title: Myopathy-causing variants of PYROXD1 converge on impaired RNA ligation Article Snippet: .. Constructs were assembled from gel-purified PCR products or from synthesized DNA fragments using Gel Purification:Article Title: A Third-generation Lentiviral Vector System for Flow Virometry-based Analysis of HIV-1 Env and Other Viral Envelope Proteins Article Snippet: .. Following DpnI digestion and gel purification of all PCR products, the fluorophore coding sequences were inserted between the duplicated HIV-1 protease cleavage sites using the Polymerase Chain Reaction:Article Title: A Third-generation Lentiviral Vector System for Flow Virometry-based Analysis of HIV-1 Env and Other Viral Envelope Proteins Article Snippet: .. Following DpnI digestion and gel purification of all PCR products, the fluorophore coding sequences were inserted between the duplicated HIV-1 protease cleavage sites using the Article Title: Myopathy-causing variants of PYROXD1 converge on impaired RNA ligation Article Snippet: .. Constructs were assembled from gel-purified PCR products or from synthesized DNA fragments using Clone Assay:Article Title: Engineering a Modular Bacterial Galactose Oxidase through CBM Recombination and Second-Shell Substitutions Article Snippet: For all PCR products, the DNA template was digested with 10 U of DpnI (Thermo Scientific) at 37 °C for 6 h, followed by purification using the Illustra GFX PCR DNA kit (GE Healthcare). .. The truncated PsGalOx variants and CBM genes were cloned into pET-15b using Molecular Cloning:Article Title: Engineering a Modular Bacterial Galactose Oxidase through CBM Recombination and Second-Shell Substitutions Article Snippet: For all PCR products, the DNA template was digested with 10 U of DpnI (Thermo Scientific) at 37 °C for 6 h, followed by purification using the Illustra GFX PCR DNA kit (GE Healthcare). .. The truncated PsGalOx variants and CBM genes were cloned into pET-15b using Plasmid Preparation:Article Title: Engineering a Modular Bacterial Galactose Oxidase through CBM Recombination and Second-Shell Substitutions Article Snippet: For all PCR products, the DNA template was digested with 10 U of DpnI (Thermo Scientific) at 37 °C for 6 h, followed by purification using the Illustra GFX PCR DNA kit (GE Healthcare). .. The truncated PsGalOx variants and CBM genes were cloned into pET-15b using Article Title: One‐Step Induction of Oogonia‐Like and Pro‐Spermatogonia‐Like Cells From Porcine Pluripotent Stem Cells Article Snippet: .. The purified target gene fragments were assembled with the linearized vector backbone using Purification:Article Title: One‐Step Induction of Oogonia‐Like and Pro‐Spermatogonia‐Like Cells From Porcine Pluripotent Stem Cells Article Snippet: .. The purified target gene fragments were assembled with the linearized vector backbone using Homologous Recombination:Article Title: One‐Step Induction of Oogonia‐Like and Pro‐Spermatogonia‐Like Cells From Porcine Pluripotent Stem Cells Article Snippet: .. The purified target gene fragments were assembled with the linearized vector backbone using |

